孙广大;李文媛;马多;王莹;

• 1:牡丹江医学院
• 2:牡丹江医学院神经组织工程研究所

摘要(Abstract)：

目的探讨转录因子KLF7对骨髓间充质干细胞(BMSCs)增殖和施万样细胞分化的作用。方法 BMSCs培养与鉴定,应用腺病毒AAV-KLF7和AAV-NC转染BMSCs。将BMSCs分为AAV-KLF7组、AAV-NC组和正常组。应用Western blots和实时荧光定量PCR(qRT-PCR)检测KLF7及其靶基因神经生长因子(NGF)表达水平,EdU免疫荧光染色及MTT法检测各组细胞增殖情况,应用施万细胞分化培养液和免疫荧光染色检测各组细胞诱导分化为类施万样细胞情况。结果与正常组和AAV-NC组比较,AAV-KLF7组KLF7蛋白和mRNA表达水平显著升高,NGF mRNA表达亦显著升高(P<0.05);AAV-KLF7组EdU阳性细胞比率显著增多,增殖活性显著增加(P<0.05);诱导10 d后AAV-KLF7组S100阳性细胞比率显著增多(P<0.05)。结论 KLF7显著促进BMSCs增殖和施万样细胞分化。

关键词(KeyWords)： KLF7;骨髓间充质干细胞;增殖;细胞分化;神经生长因子

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(编号:81870977);; 黑龙江省自然科学基金项目(编号:H2018068);; 黑龙江省卫生计生委立项科研课题(编号:2018-379);; 黑龙江省属高校基本科研业务费项目(编号:2019-KYYWFMY-0018);; 牡丹江医学院研究生创新科研项目(编号:2019YJSCX-06MY)

作者(Authors): 孙广大;李文媛;马多;王莹;

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