禚志红;张静;李国栋;王怀立;

• 1:郑州大学第一附属医院儿科
• 2:郑州大学生命科学学院

摘要(Abstract)：

目的探讨脂多糖(LPS)预处理对幼鼠癫痫发作及海马炎症介质Toll样受体4/高迁移率族蛋白1/磷酸化核因子抑制蛋白α(TLR4/HMGB1/P-IκB-α)表达的影响。方法出生21d的SD鼠随机分为生理盐水组(对照组),模型Ⅰ组和模型Ⅱ组,模型Ⅰ组采用海人酸(KA)诱导癫痫发作,模型Ⅱ组在应用KA前2h腹腔注射LPS,观察幼鼠癫痫发作的行为学表现,荧光定量PCR检测癫痫持续状态(SE)后3h和24h各组海马TLR4和HMGB1基因的表达,Westernblot法检测SE后3h、24h各组海马TLR4、HMGB1、P-IκB-α蛋白的表达。结果与对照组相比:模型Ⅰ组、模型Ⅱ组海马TLR4基因在SE后3h表达均显著增加(t=4.806,P<0.05;t=4.954,P<0.05),SE后24h也显著增加(t=3.924,P<0.05;t=3.792,P<0.05),模型Ⅰ组、模型Ⅱ组海马TLR4蛋白表达在SE后3h均显著增加(t=7.804,P<0.05;t=8.385,P<0.05),SE后24h也显著增加(t=4.256,P<0.05;t=4.262,P<0.05);模型Ⅰ组、模型Ⅱ组海马HMGB1基因在SE后3h表达均显著增加(t=3.626,P<0.05;t=5.255,P<0.05),SE后24h也显著增加(t=4.046,P<0.05;t=2.836,P<0.05),模型Ⅰ组、模型Ⅱ组海马HMGB1蛋白在SE后3h均无显著性增加(t=0.389,P>0.05;t=0.213,P>0.05),SE后24h也无显著性增加(t=0.106,P>0.05;t=0.279,P>0.05)。模型Ⅰ组、模型Ⅱ组海马P-IκB-α蛋白表达在SE后3h均显著增加(t=4.383,P<0.05;t=6.627,P<0.05),SE后24h也显著增加(t=14.521,P<0.05;t=19.458,P<0.05)。模型Ⅱ组与模型Ⅰ组相比,LPS预处理可显著增加海马TLR4基因在SE后3h的水平(t=2.362,P<0.05),及SE后3hTLR4蛋白表达(t=4.284,P<0.05);且显著增加SE后3h、24hPIκB-α蛋白表达(t=4.249,P<0.05;t=9.120,P<0.05);但对SE后3h、24hHMGB1基因水平无显著性影响(t=0.569,P>0.05;t=0.691,P>0.05),对SE后3h、24hHMGB1蛋白表达也无显著性影响(t=0.168,P>0.05;t=0.385,P>0.05)。结论LPS预处理加重幼鼠癫痫发作,使TLR4/P-IκB-α表达升高,对HMGB1表达无显著改变。

关键词(KeyWords)： 脂多糖;海人酸;癫痫;高迁移率族蛋白1;Toll样受体4;P-IκB-α;大鼠

Abstract:

Keywords:

基金项目(Foundation): 河南省高等学校重点科研项目计划(13A320433);; 郑州大学第一附属医院“院内青年创新基金计划项目”

作者(Authors): 禚志红;张静;李国栋;王怀立;

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