MCT1 MMP-2mRNA在人脑胶质瘤的表达及相关性研究
寿记新,高海东,王建业,刘菲菲,付旭东,王酩,丁攀峰,孟恩平,周少龙
摘要(Abstract)：
目的探讨单羧酸转运蛋白-1(MCT1)和基质金属蛋白酶2(MMP-2)mRNA在人脑胶质细胞瘤中的表达及相关性。方法应用荧光定量qRT-PCR法,分别测定50例原发性人脑胶质细胞瘤和10例脑内减压术患者脑组织标本中MCT1、MMP-2mRNA表达水平。结果 (1)MCT1、MMP-2在人脑胶质细胞瘤组织中mRNA表达较对照组显著增强(P<0.05);(2)MCT1、MMP-2在高级别胶质瘤组较低级别胶质瘤组mRNA表达显著增加(P<0.05);(3)MCT1、MMP-2mRNA在胶质瘤中的表达可能具有正相关性(r=0.712,P=0.001),对照组标本中均未见明显MCT1、MMP-2mRNA表达。结论(1)MCT1、MMP-2mRNA的表达同胶质瘤恶性级别关系密切,且病理级别越高其表达强度明显增强;(2)MCT1、MMP-2mRNA表达可能协同参与了人脑原发性胶质瘤的病理发展过程;(3))MCT1、MMP-2mRNA关联检测有可能作为判断胶质瘤侵袭性及其预后的重要参考指标。
关键词(KeyWords)： 单羧酸转运蛋白-1;基质金属蛋白酶-2;脑胶质瘤
基金项目(Foundation): 河南省科技厅项目(102300410065)
作者(Author): 寿记新,高海东,王建业,刘菲菲,付旭东,王酩,丁攀峰,孟恩平,周少龙
参考文献(References)：
- [1]程森,寿记新,付旭东,等.NS和PUMA蛋白在人脑胶质瘤中的表达及其临床意义[J].中华神经医学杂志,2014,13(12):1 256-1 259.
- [2]彭苗,丁颖,刘晓清,等.脑胶质瘤病22例临床观察和分析[J].实用医学杂志,2012,28(15):2 570-2 572.
- [3]韩松,董韬,于春泳,等MCT1CD147在人胶质瘤中的表达与预后的相关性[J]中国实用神经疾病杂志2014,17(1):1-3.
- [4]Miranda-Gonalves V,Honavar M,Pinheiro C,et al.Monocarbox-ylate transporters(MCTs)in gliomas:expression and exploitationas therapeutic targets.Neuro Oncol,2013,15(2):172-188.
- [5]Dixit D,Sharma V,Ghosh S,et al.Inhibition of Casein kinase-2induces p-53-dependent cell cycle arrest and sensitizes glioblastoma cells to tumor necrosis factor(TNFa)-induced apoptosis through SIRT1inhibition[J].Cell Death Dis,2012,9(3):271.
- [6]Halestrap AP.The SLC16gene family-structure,role and regulation in health and disease.Mol Aspects Med,2013,34(2-3):337-349.
- [7]张宝月,瞿全新,顾安康,等CD147、MCT1及MCT4在宫颈病变中的表达及临床意义[J]国际妇产科学杂志2015,(42)2:194-196.
- [8]De Saedeleer CJ,Copetti T,Porporato PE,et al.Lactate activatesHiF-1in oxidative but not in Warburg-phenotype human tumorcells.PLoS One,2012,7(10):e46 571.
- [9]Nima-Etminan D.Corinna-Peters SC,Ficnar J,et al.Modulation of migratory activity and invasiveness of human glioma spheroids following S-aminolevulinic acid based photodynamic treatment[J].Neurosurgery,2011,115(2):281-288.
- [10]Hisaoka K,Tsuchioka M,Yano R,et al.Tricyclic antidepressant amitriptyline Activates fibroblast growth factor receptor signaling in glial cells:involvement in glial cell lined erived neuro trophic factor production[J].BidChem,2011,286(10):21 118-21 108.
- [11]Chen Y,Tsai YH,Tseng SH.Valproic acid affected the survival and invasiveness of human glioma cells through diverse mechanisms[J].ournal of Neuro-Oncology,2012,109(1):23-33.
- [12]Badiga AV,Chetty C,Kesanakurti D,et al.MMP-2siRNA Inhibits Radiation-Enhanced Invasiveness in Glioma Cells[J].PLoS One,2011,6(6):20 614.