刘方;张敏;刘恒方;刘田田;

• 1:郑州大学第五附属医院
• 2:郑州大学第一附属医院

摘要(Abstract)：

目的观察丙戊酸钠对氯化锂-匹罗卡品癫痫鼠模型海马区B1及B2激肽受体(KB2R)表达的影响并探讨其作用机制。方法健康Wistar大鼠35只,采用氯化锂-匹罗卡品腹腔注射法制作癫痫模型,癫痫模型干预组15只,分为急性期丙戊酸钠干预组(12 h,Ⅰ组)、静止期丙戊酸钠干预组(5 d,Ⅱ组)、慢性期丙戊酸钠干预组(60 d,Ⅲ组),每组5只;癫痫模型无干预组15只,分为急性期无干预组(12 h,Ⅰa组),静止期无干预组(5 d,Ⅱa组),慢性期无干预组(60 d,Ⅲa组),每组5只;设置空白对照组5只(Ⅳ组)。丙戊酸钠均经鼻饲管给药,在干预后的相应时间点处死动物取海马标本,应用逆转录聚合酶链反应(RT-PCR)及western blot方法分别检测各组动物不同时间点海马区的KB1R及KB2R的表达变化,并相互进行比较。结果给予丙戊酸钠干预后,Ⅰ组与Ⅰa组比较,Ⅰ组KB1R mRNA及蛋白质表达均显著下调,差异具有显著性(P<0.05),Ⅰ组KB2R mRNA及蛋白质表达均显著上调,差异具有显著性(P<0.05);Ⅱ组与Ⅱa组比较,Ⅱ组KB1R mRNA及蛋白质表达显著下调,差异具有显著性(P<0.05);Ⅲ组与Ⅲa组比较,Ⅲ组KB2R mRNA及蛋白质表达均显著上调,差异具有显著性(P<0.05);癫痫模型无干预组与空白对照组比较,Ⅰa组及Ⅱa组KB1R及KB2R及蛋白质表达均显著上调,差异具有显著性(P<0.05),Ⅲa组KB2R及蛋白质表达均显著上调,差异具有显著性(P<0.05)。结论 KB1R在癫痫形成过程中对脑组织可能具有兴奋性细胞毒作用,KB2R可能对海马组织有保护作用,丙戊酸钠抗癫痫的干预实验也支持这个观点。

关键词(KeyWords)： B激肽受体;丙戊酸钠;匹罗卡品;癫痫模型

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(编号:81901326)

作者(Author): 刘方;张敏;刘恒方;刘田田;

Email:

DOI:

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