苗亚飞;杨木强;司马靓杰;闫俊强;

• 1:河南科技大学第一附属医院

摘要(Abstract)：

目的观察七氟醚麻醉干预后脑缺血再灌注损伤大鼠的学习记忆能力改变以及海马S100β和颗粒蛋白前体(PGRN)的表达变化。方法选取雄性SD大鼠30只,随机分为假手术组(Sham)、缺血再灌注损伤组(CIRI)、七氟醚后处理组(SEVO),每组10只。CIRI组和SEVO组大鼠采用线栓法制作脑缺血再灌注损伤模型,Sham组大鼠仅切开不做线栓处理,SEVO组大鼠于再灌注后给予3%七氟醚和氧气混合吸入30 min,氧流量1 L/min,Sham组及CIRI组则单纯给予相同流量的氧气吸入。24 h后使用改良大鼠神经功能缺损评分(mNSS)评估各组大鼠的神经功能,采用Morris水迷宫检测各组大鼠学习记忆能力改变,Western blot检测各组大鼠海马S100β及PGRN的表达改变。结果 CIRI组及SEVO组mNSS评分均明显增高(P<0.001),同时SEVO组mNSS评分相对于CIRI组明显下降(P<0.001);TTC结果显示SEVO组大鼠梗死体积百分比显著降低(P<0.001);水迷宫试验显示SEVO组大鼠逃逸潜伏期明显缩短(P<0.05);Western结果显示SEVO组大鼠S100β水平明显降低(P<0.05),PGRN水平显著上升(P<0.01)。结论七氟醚后处理可明显改善CIRI大鼠学习记忆能力损害与脑梗死程度,七氟醚可能通过降低S100β及调高PGRN表达水平发挥神经保护作用。

关键词(KeyWords)： 缺血再灌注损伤;脑梗死;七氟醚;S100β;颗粒蛋白前体;学习记忆;海马;SD大鼠

Abstract:

Keywords:

基金项目(Foundation): 河南省自然科学基金(编号:182300410333);; 河南科技大学青年科学基金项目(编号:2015QN047)

作者(Author): 苗亚飞;杨木强;司马靓杰;闫俊强;

Email:

DOI:

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