HOTAIR通过E-cadherin对胶质瘤侵袭和转移的影响研究Study of HOTAIR effect invasion and metastasis of glioma by E-cadherin
李锡清;赵尊兰;刘冬玲;刘明月;马宁;李明;周云;
摘要(Abstract)：
目的观察HOTAIR对胶质瘤细胞侵袭和转移能力的影响。方法使用q-PCR从临床标本中检测HOTAIR与E-cadherin的关系,体外常规培养SWO-38和U251细胞,过表达HOTAIR后使用划痕实验检测细胞侵袭、迁移能力;Western blotting检测细胞中E-cadherin蛋白的表达。结果 q-PCR胶质瘤组织中HOTAIR高表达组中E-cadherin表达的均值较低,较HOTAIR低表达组低,差异有统计学意义(P<0.05);在SWO-38和U251细胞中过表达HOTAIR后Western blotting检测发现E-cadherin表达减少,划痕实验显示过表达HOTAIR后细胞迁移能力增强。结论 HOTAIR可增加胶质瘤的侵袭和转移,可能与其降低E-cadherin蛋白的表达有关。
关键词(KeyWords)： 胶质瘤;HOTAIR;E-cadherin侵袭
基金项目(Foundation): 国家自然科学基金(U1504820)
作者(Authors): 李锡清;赵尊兰;刘冬玲;刘明月;马宁;李明;周云;
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