刘庆杰;白宏英;

• 1:永城市人民医院神经内科
• 2:郑州大学第二附属医院神经内科

摘要(Abstract)：

目的探讨乌司他丁对脑缺血再灌注大鼠缺血侧脑组织c-Jun氨基末端激酶(JNK)蛋白表达及凋亡细胞数的影响。方法 36只雄性清洁SD大鼠按随机平均原则分成3组:假手术组(12只)、脑缺血再灌注组(对照组,12只)、脑缺血再灌注+乌司他丁治疗组(治疗组,12只)。大鼠局灶性脑缺血再灌注损伤模型采用大脑中动脉线栓法制作。采用RT-PCR法检测大鼠脑组织JNK的表达,采用TUNEL法检测大鼠脑组织凋亡细胞数。结果与假手术组相比,对照组和治疗组大鼠脑组织皮质区JNK的表达明显升高,凋亡细胞数均明显增加(P均<0.05)。与对照组相比,治疗组大鼠脑组织JNK的表达明显下降,凋亡细胞数均明显减少(均P<0.05)。结论乌司他丁可下调缺血再灌注大鼠脑组织JNK的表达并抑制其细胞凋亡,乌司他丁抑制细胞凋亡可能与抑制JNK传导通路相关。

关键词(KeyWords)： 乌司他丁;脑缺血再灌注;c-Jun氨基末端激酶;细胞凋亡

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 刘庆杰;白宏英;

参考文献(References)：

• [1]Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
• [2]Chekeni FB,Elliott MR,Sandilos JK,et al.Pannexin 1channels mediate'find-me'signal release and membrane permeability during apoptosis[J].Nature,2010,467(7 317):863-867.
• [3]Svensson C,Part K,Künnis-Beres K,et al.Pro-survival effects of JNK and p38MAPK pathways in LPS-induced activation of BV-2cells[J].Biochem Biophys Res Commun,2011,406(3):488-492.
• [4]Xie P,Guo S,Fan Y,et al.Atrogin-1/MAFbx enhances simulated ischemia/reperfusion-induced apoptosis in cardiomyocytes through degradation of MAPK phosphatase-1 and sustained JNK activation[J].Biol Chem,2009,284(9):5 488-5 496.
• [5]Song JJ,Lee YJ.Dissociation of Akt1from its negative regulator JIP1is mediated through the ASK1-MEKJNK signal transduction pathway during metabolic oxidative stress:a negative feedback loop[J].Cell Biol,2005,170(1):61-72.
• [6]Nakamagoe M,Tabuchi K,Uemaetomari I,et al.Estradiol protects the cochlea against gentamicin ototoxicity through inhibition of the JNK pathway[J].Hear Res,2010,261(1/2):67-74.
• [7]Schwertassek U,Buckley DA,Xu CF,et al.Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase(JNK)stimulatory phosphatase 1is necessary for its activation of JNK signaling and apoptosis[J].FEBS J,2010,277(11):2 463-2 473.
• [8]Bivik C,Ollinger K.JNK mediates UVB-induced apoptosis upstream lysosomal membrane permeabilization and Bcl-2family proteins[J].Apoptosis,2008,13(9):1 111-1 120.