董真真;袁峰;白倩;

• 1:郑州大学第二附属医院

摘要(Abstract)：

目的探索乌司他丁对脑缺血再灌注大鼠海马N-甲基-D-天门冬氨酸受体(NMDAR)表达的影响。方法将72只雄性SD大鼠随机分为假手术组(S组)、模型组(I/R组)和乌司他丁干预组(U组)。采用线栓法阻断大脑右侧中动脉建立脑缺血损伤模型。U组于再灌注即刻腹腔注射乌司他丁20 000 U/kg,再灌注3 h后进行神经功能评分(NDS),采用TUNEL法检测脑组织凋亡细胞数,RT-PCR检测NMDAR1 mRNA的表达水平,采用免疫组化染色计算平均光密度值(MOD)观察NMDAR1的表达。结果与S组(0)比较,I/R组(2.58±0.35)和U组(1.34±0.41)的NDS升高(P<0.05);与S组(0)比较,I/R组(52.10±3.25)和U组(26.40±2.70)的凋亡细胞数增加(P<0.05);与S组(0.890 2±0.013 6)比较,I/R组(1.039 8±0.021 1)和U组(0.907 3±0.014 2)NMDAR1 mRNA的表达上调(P<0.05);与S组(0.006 6±0.000 7)比较,I/R组(0.059 4±0.011 1)和U组(0.006 8±0.000 4)NMDAR1表达的MOD值升高(P<0.05)。与I/R组(2.58±0.35)比较,U组(1.34±0.41)的NDS降低(P<0.05);与I/R组(52.10±3.25)比较,U组(26.40±2.70)凋亡细胞数减少(P<0.05);与I/R组(1.039 8±0.021 1)比较,U组(0.907 3±0.0142)NMDAR1 mRNA的表达下降(P<0.05);与I/R组(0.059 4±0.011 1)比较,U组(0.006 8±0.000 4)NMDAR1表达的MOD值降低(P<0.05)。结论乌司他丁减轻脑缺血再灌注损伤的机制可能与抑制NMDAR1表达有关。

关键词(KeyWords)： 脑缺血再灌注损伤;乌司他丁;兴奋性氨基酸;N-甲基-D-天门冬氨酸受体;海马组织;SD大鼠

Abstract:

Keywords:

基金项目(Foundation): 河南省优秀青年人才培养项目（编号：YXKC2020059）

作者(Authors): 董真真;袁峰;白倩;

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