生长抑素受体1在皮质发育障碍大鼠海马的异常表达Abnormal expression of somatostatin receptor 1 in the hippocampus of the disorders of cortical development rat models
张建刚,宋延波,张毅,贺兴,冯飞,晏勇
摘要(Abstract)：
目的通过研究生长抑素受体1(sstr-1)在皮质发育障碍模型大鼠海马区中的表达,探讨皮质发育障碍模型大鼠海马形态改变的发生机制及致癫机制。方法制作皮质发育障碍大鼠模型,选取P60 d仔鼠,利用免疫组化、Western Blot检测sstr-1在皮质发育障碍模型大鼠海马区的变化。结果与正常对照组比较,sstr-1在皮质发育障碍模型大鼠海马区水平表达与正常对照组相比明显降低(0.59±0.15 vs 1.02±0.14,P<0.052)。结论 sstr-1在皮质发育障碍大鼠模型海马神经元的迁移紊乱及致癫机制中具有重要作用。
关键词(KeyWords)： 皮质发育障碍;海马;生长抑素受体1
基金项目(Foundation):
作者(Author): 张建刚,宋延波,张毅,贺兴,冯飞,晏勇
参考文献(References)：
- [1]Kuzniecky RI,Jackson GD.Magnetic resonance in epilepsy[J].New York:Raven Press,1995:183-202.
- [2]Leventer RJ,Phelan EM,Coleman LT,et al.Clinical and im-aging features of cortical malformations in childhood[J].Neu-rology,1999,53:715-722.
- [3]Galileo D,Majors J,Horwitz A,et al.Retrovirally introducedantisense integrin RNA inhibits neuroblast migration in vivo[J].Neuron,1992,9:1 117-1 131.
- [4]Nakanishi K,Watanabe K,Kawabata M,et al.Altered syn-aptic activities in cultures of neocortical neurons from prenatal-ly X-irradiated rats[J].Neurosci Lett,2004,355(1/2):61-64.
- [5]Okazaki Y,Furuno M,Kasukawa T,et al.Analysis of themouse transcriptome based on functional annotation of 60,770full-length cDNAs[J].Nature,2002,420(6915):563-573.
- [6]Fodor M,van Elk EJ,Huizinga CT,et al.NPY neurons expresssomatostatin receptor subtype 1 in the arcuate nucleus[J].Neuroreport,2005,16(1):29-32.
- [7]Tannenbaum GS,Zhang WH,Lapointe M,et al.Growth hor-mone-releasing hormone neurons in the arcuate nucleus expressboth sst1 and sst2 somatostatin receptor genes[J].Endocrinol-ogy,1998,139:1 450-1 453.
- [8]Watt HL,Kumar U.Colocalization of somatostatin receptorsand epidermal growth factor receptors in breast cancer cells[J].Cancer Cell Int,2006,6(1):5.
- [9]Wang XP,Norman M,Yang J,et al.Alterations in glucose ho-meostasis in sstr-1 gene-ablated mice[J].Mol Cell Endocrinol,2006,247(1/2):82-90.
- [10]Cammalleri M,Martini D,Timperio AM,et al.Functionaleffects of somatostatin receptor 1 activation on synaptic trans-mission in the mouse hippocampus[J].J Neurochem,2009,111(6):1 466-1 477.
- [11]Cammalleri M,Cervia D,Langenegger D,et al.Somatostatinreceptors differentially affect spontaneous epileptiform activityin mouse hippocampal slices[J].Eur J Neurosci,2004,20(10):2 711-2 721.