苏淑杰,李志洁

• 1:郑州大学第二附属医院

摘要(Abstract)：

目的探讨鼠神经生长因子(NGF)对新生大鼠缺氧缺血性脑损伤(HIBD)后神经修复的作用。方法新生7日龄(SD)大鼠72只随机分成假手术组、模型组、治疗组,模型组腹腔注射生理盐水,治疗组腹腔注射NGF,采用免疫组化检测术后不同时间点(3 h、12 h、1 d、3 d、7 d、14 d)血管内皮生长因子(VEGF)及神经丝蛋白(NFP)的表达。结果假手术组VEGF呈持续的低程度表达,HIBD后治疗组各时间点VEGF的表达均高于模型组,3 h~3 d治疗组的VEGF阳性区平均积分光密度(IOD)值大于模型组(P<0.05),且高峰提前至3 h,2组间7 d及14 d VEGF表达无统计学差异。HIBD后,3 h~1 d模型组和治疗组的NFP阳性区平均IOD值均显著低于假手术组(P<0.01),3 d、7 d及14 d治疗组NFP阳性区平均IOD值显著高于模型组(P<0.01)。结论 NGF对HIBD的神经修复机制之一,可能是通过上调VEGF及NFP的表达而实现。

关键词(KeyWords)： 神经生长因子;缺氧缺血性脑损伤;血管内皮生长因子;神经丝蛋白

Abstract:

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基金项目(Foundation):

作者(Author): 苏淑杰,李志洁

参考文献(References)：

• [1]田培超,陈国洪.108例新生儿缺氧缺血性脑病诊治分析[J].中国实用神经疾病杂志,2008,11(5):36-38.
• [2]Ki m DH,Zhao XR,Tu CH,et al.Prevention of apoptotic butnot necrotic cell death following neuronal injury by neurotro-phins signaling through the tyrosine kinase receptor[J].J Neu-rosurg,2004,100(1):79-87.
• [3]Tang XQ,Cai J,Nelson KD,et al.Functional repair after dorsalroot rhizotomy using nerve conduits and neurotrophic molecules[J].Eur J Neurosci,2004,20(5):1 211-1 218.
• [4]Wang YM,Jin K,Mao XO,et al.VEGF-overexpressing trans-genic mice show enhance post-ischemic neurogenesis and neu-romigration[J].J Neurosci Res,2007,85(4):740-747.
• [5]Kiselyov A,Balakin KV,Tkachenko SE.VEGF/VEGFR sig-nalling as a target for inhibiting angiogenesis[J].Expert OpinInvestig Drugs,2007,16(1):83-107.
• [6]Wang YM,Kilic E,Kilic U,et al.VEGF overexpression in-duces post-ischaemic neuroprotection,but facilitates haemody-namic steal phenomena[J].Brain,2005,128(1):52-63.
• [7]Letournel F,Bocquet A,Perrot R,et al.Neurofilament highmolecular weight-green fluorescent protein fusion is normallyexpressed in neurons and transported in axons:A neuronal-marker toinvestigate the biology of neurofilaments[J].Neuro-science,2006,137(1):103-111.
• [8]Gama SM,Friedrich V,DeGasperi R,et al.Human midsizeneurofilament subunit induces motor neuron disease in trans-genic mice[J].Exp Neurol,2003,184(1):408-419.
• [9]Grosjean J,Kiriakidis S,Reilly K,et al.Vascular endothelialgrowth factor signallingin endothelial cell survival:Arole forNFκB[J].Biochem Biophys Res Communol,2006,340(3):984-994.
• [10]Liu Q,Xie F,Siedlak SL,et al.Neurofilament proteins in neu-rodegenerative diseases[J].Cell Mol Life Sci,2004,61(24):3057-3 075.