乳胞素对SD大鼠脑出血模型神经新生的影响及机制Effect of lactacystin on nerve newborn ability of intracerebral hemorrhage model and its mechanism
陈勇,高林,耿利娇,贺维亚
摘要(Abstract)：
目的探讨UPS抑制剂乳胞素(lactacystin)对SD大鼠脑出血模型神经新生的影响及机制。方法采用Ⅶ型胶原酶诱导SD大鼠脑出血模型,分为对照组(A组),乳胞素处理组(B组),每组各20只;造模成功后第1~7天B组腹腔注射1mg/(kg.d)的乳胞素,A组腹腔注射等剂量生理盐水。第3周断头处死SD大鼠,以组织化学方法结合灰度值半定量检测泛素-蛋白酶体系统活性表达,采用分光光度计测定法检测转录因子-核因子-κB(nuclear factor-κB,NF-κB)含量,利用抗Brdu单克隆抗体,显示纹状体区神经前体细胞增殖的能力。结果与A组比较,B组泛素-蛋白酶体系统活性明显下降,损毁侧纹状体内NF-κB含量含量明显减少,神经前体细胞增殖的能力增强(P<0.01)。结论乳胞素有利于SD大鼠脑出血模型神经新生,其机制可能与抑制泛素-蛋白酶体,调控NF-κB含量,减少神经元凋亡,促进神经元新生有关。
关键词(KeyWords)： 乳胞素;泛素-蛋白酶体系统;转录因子-核因子-κB;脑出血模型
基金项目(Foundation):
作者(Author): 陈勇,高林,耿利娇,贺维亚
参考文献(References)：
- [1]Sterzi R,Vidale S.Treat ment of intracerebral hemorrhage:theclinical evidence[J].Neurol Sci,2004,25(Suppl 1):S12.
- [2]Larive LL,Carhuapoma JR.Perihematoma brain metabolismand edema:thus far,an elusive piece of a complex puzzle[J].JNeurol Sci,2006,224(1):1-2.
- [3]Di Napoli M,Papa F.The proteasome system and proteasomeinhibitors in stroke:controlling the inflammatory response[J].Curr Opin Invest Drug,2003,4(11):1 333-1 342.
- [4]Reinstein E.I mmunologic aspects of protein degradation by theubiquitin-proteasome system[J].Isr Med Assoc J,2007,6(7):420-424.
- [5]张海娜,胡国华,陈秋惠,等.乳胞素诱导大鼠致帕金森病的实验研究[J].中国老年学杂志,2009,18(2):15-17.
- [6]Lee MH,Hyun DH,Jenner P,et al.Effect of proteasome inhi-bition on cellular oxidative damage,antioxidant defences and ni-tric oxide production[J].J Neurochem,2001,78(1):32-41.
- [7]McNaught KS,Olanow CW,Halli wel1 B,et al.Failure of theubiquitin-proteasome systemin Parkinson’s disease[J].NatRev Neurosci,2001,2(8):589-594.