一氧化氮与一氧化氮合酶抑制剂对脑缺血后海马神经元的保护作用Neuroprotective effect of sodium nitroprusside and nitric oxide synthase inhibitors on rat hippocampal CA1 neurons against cerebral ischemia
胡文忠,王晓强
摘要(Abstract)：
目的研究硝普钠、7-硝基吲唑(7-NI)和AMT对急性脑缺血后海马神经元细胞是否具有保护作用。方法将80只SD大鼠随机分组,按照不同给药时间和给药方法制作全脑缺血再灌注模型,待全脑缺血15 min后再灌注,第5天行多聚甲醛灌注,石蜡包埋,尼氏染色观察海马CA1区神经元的存活情况。结果在脑缺血再灌注后5 d,单次应用硝普钠未见明显保护作用,连续应用硝普钠7次和应用AMT和7-NI组存活率较未给药组明显增多,尼氏染色显示海马神经元细胞的存活率较对照组明显增多。结论硝普钠、7-NI和AMT均对急性脑缺血后海马神经元具有重要的保护作用。
关键词(KeyWords)： 硝普钠;一氧化氮合酶抑制剂;神经元;脑缺血/再灌注;脑保护
基金项目(Foundation):
作者(Author): 胡文忠,王晓强
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