慢性脑缺血老龄大鼠海马区突触素及NMDAR2B表达的研究Research of synaptophysin and NMDAR2B in hippocampus of aged rats after chronic cerebral hypoperfusion
郭铁
摘要(Abstract)：
目的本实验旨在观察慢性脑缺血老龄大鼠海马区突触素及NR2B表达的变化。方法健康Wistar大鼠100只,随机分为5组,A组(对照组)、B组(缺血4周组)、C组(缺血8周组)、D组(缺血12周组)、E组(缺血16周组),每组20只。造模方法采用手术结扎大鼠双侧颈总动脉。分别于手术后4周、8周、12周、16周,采用免疫组化方法观察慢性脑缺血老龄大鼠海马区NR2B及突触素表达的变化。结果各组间海马区神经元突触素及NR2B表达水平的比较显示,缺血第4、8、12及16周组突触素及NR2B灰度值分别低于对照组(P<0.05);缺血第12周及第16周组还分别低于缺血第4、8周组,差异均有统计学意义(P<0.05)。结论慢性脑缺血后第4、8、12、16周,老龄大鼠海马结构内CA1区、CA3区及齿状回内突触素和NR2B表达明显逐渐减少。
关键词(KeyWords)： 慢性脑缺血;NMDA受体;NR2B;突触素
基金项目(Foundation):
作者(Author): 郭铁
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