诱导型一氧化氮合酶及缺氧诱导因子-1α在人脑胶质瘤的表达意义与肿瘤血管生成关系的研究Study of the expression of inducible nitric oxide synthase and hypoxia-inducible factor-1 alpha in human gliomas and the relationship between them and the formation of tumor vessel
胡伟鑫,陈春美,石松生,杨卫忠,李绍熹,叶金练
摘要(Abstract)：
目的探讨诱导型一氧化氮合酶(iNOS)、缺氧诱导因子-1α(HIF-1α)在人脑胶质瘤标本表达特点及相关性,探讨HIF-1α和iNOS与肿瘤血管形成的相关性。方法收集86例人脑胶质瘤(低度恶性组42例,高度恶性组44例)和新鲜脑组织10例(对照组),应用免疫组化SP法测定HIF-1αi、NOS和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达水平并分析其相关性。结果免疫组化结果显示,iNOS在对照组细胞中不表达,低度恶性组胞浆中呈阳性表达,而高度恶性组呈强阳性表达,2组之间表达强度差异具有统计学意义(P<0.05);HIF-1α和VEGF在正常脑组织组和胶质瘤细胞中均有表达,其中正常脑组织均弱阳性表达,低度恶性组均呈阳性表达,而高度恶性组均呈强阳性表达,HIF-1α和VEGF在3组之间表达强度差异均具有统计学意义(P<0.05)。根据免疫组化结果进行各种抗原表达相关性分析,HIF-1αi、NOS和VEGF在低度恶性组和高度恶性组中表达均具有相关性。结论 HIF-1αi、NOS和VEGF共同参与胶质瘤细胞发生发展过程,三种基因表达强度均与胶质瘤细胞恶性度相关,恶性度越高,表达量越多;HIF-1αi、NOS和VEGF三者可能存在相互协调关系,共同促进胶质瘤生长增殖和转移过程。
关键词(KeyWords)： 诱导型一氧化氮合酶;缺氧诱导因子-1α;血管内皮生长因子;胶质瘤;肿瘤血管
基金项目(Foundation): 福建省教育厅科技计划项目(JA05259)
作者(Author): 胡伟鑫,陈春美,石松生,杨卫忠,李绍熹,叶金练
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