王浆蛋白质及其生理药理作用分析Analysis of royal jelly proteome and its physiological and pharmacological function
王婷,杨旭
摘要(Abstract):
目的从蛋白质组的角度分析王浆及其生理药理作用,为进一步理解王浆医用价值提供理论基础。方法提取王浆全蛋白质组分进行双向电泳(2-DE)和质谱鉴定(MALDI-TOF/MS),然后利用蛋白质数据库进行比对。结果 2-DE图谱共检测出152个蛋白点,对其中57个高丰度蛋白进行了MALDI-TOF/MS鉴定,有45个属于王浆主蛋白(MRJPs)家族,还有3个葡萄糖氧化酶,1个过氧化物还原酶,1个谷胱甘肽S转移酶,其余7个蛋白点未能找出匹配蛋白。结论这些蛋白质和酶类具有营养、免疫调节、抗氧化、抗炎、抗肿瘤等多种生物活性,或许可以部分说明王浆对于人类的医药保健作用。
关键词(KeyWords): 王浆;蛋白质组;医药保健
基金项目(Foundation):
作者(Author): 王婷,杨旭
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