人胚胎干细胞神经分化中VEGF作用的分子机制研究Molecular mechanism of VEGF on neural differentiation of human embryonic stem in vitro
焦淑洁,许慧芳,张苏明,许杰,湛延强
摘要(Abstract)：
目的观察血管内皮生长因子(vascular endothelial growth factor,VEGF)对人胚胎干细胞分化为神经元的促进作用是否与p38MAPK信号通路相关。方法人胚胎干细胞经拟胚体向神经元分化,分为3组:A组:常规诱导组;B组:常规诱导+VEGF(10ng/mL)作用组;C组:常规诱导+p38MAPK抑制剂预处理+VEGF(10ng/mL)作用组。免疫荧光法检测计算各组不同阶段细胞阳性率,半定量RT-PCR观察各组神经干细胞p38αmRNA表达,MTT法检测3组神经干细胞增殖速率。结果免疫荧光法检测,B、C 2组产生神经干细胞的阳性率明显高于A组,差异有统计学意义(P<0.01),B、C 2组比较差异无统计学意义(P>0.05);B组进一步分化为神经元的阳性率明显高于A、C组,差异有统计学意义(P<0.01),A、C 2组比较差异无统计学意义(P>0.05);半定量RT-PCR观察B组神经干细胞p38αmRNA表达明显高于A、C 2组;MTT法检测与半定量RT-PCR结果相似。结论人胚胎干细胞体外分化过程中,血管内皮生长因子促进神经干细胞分化的作用与p38MAPK通路无关,而VEGF通过激活p38MAPK信号通路,促进神经干细胞进一部分化为神经元。
关键词(KeyWords)： 人胚胎干细胞;神经分化;血管内皮生长因子;p38MAPK信号通路
基金项目(Foundation):
作者(Author): 焦淑洁,许慧芳,张苏明,许杰,湛延强
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