顾冰

• 1:解放军第91中心医院心内科

摘要(Abstract)：

目的探讨原花青素(PC)在D-半乳糖致衰老大鼠大脑、心脏组织中的抗氧化作用。方法取健康6月龄Wist-ar大鼠50只,雌雄各半,分性别随机均分为正常对照组、衰老模型组、维生素E(VE)组、PC 120mg/kg和60mg/kg 2个剂量组等5组(各10只),分别给予相应的处理。测定各组大鼠大脑、心脏组织中的超氧化物歧化酶(SOD)水平、丙二醛(MDA)水平及谷胱甘肽过氧化物酶(GSH-Px)水平。结果衰老模型组较正常对照组大鼠大脑、心脏组织中的SOD水平降低、MDA水平升高、GSH-Px水平降低(P<0.01),各用药组较衰老模型组大鼠大脑、心脏组织中的SOD水平升高、MDA水平降低、GSH-Px水平升高(P<0.01),PC 120mg/kg组与VE组的三者比较差异无统计学意义(P>0.05),PC 60mg/kg组与其他2个用药组的三者比较差异有统计学意义(P<0.01)。结论 PC对D-半乳糖致衰老大鼠大脑、心脏组织具有抗氧化作用,可能具有延缓衰老的作用。

关键词(KeyWords)： 原花青素;衰老;超氧化物歧化酶;丙二醛;谷胱甘肽过氧化物酶

Abstract:

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作者(Author): 顾冰

参考文献(References)：

• [1]Reiter RJ,Paredes SD,Korkmaz A,et al.Melatonin in rela-tion to the"strong"and"weak"versions of the free radicaltheory of aging[J].Adv Med Sci,2008,53(2):119-129.
• [2]Molan AL,De S,Meagher L.Antioxidant activity and poly-phenol content of green tea flavan-3-ols and oligomeric proan-thocyanidins[J].Int J Food Sci Nutr,2008,12:1-10.
• [3]Shahat AA,Cos P,De Bruyne T,et al.Antiviral and antioxi-dant activity of flavonoids and proanthocyanidins from Cratae-gus sinaica[J].Planta Med,2002,68(6):539-541.
• [4]Rigalleau V,Beylot M,Normand S,et al.Mechanism of in-creased plasma glucose levels after oral glucose ingestion innormal-weight middle-aged subjects[J].Ann Nutr Metab,2003,47(5):186-193.
• [5]邹承鲁.当代生物学[M].北京:中国致公出版社,2000:394-396.
• [6]Li XM,Shi YH,Wang F,et al.In vitro free radical scavengingactivities and effect of synthetic oligosaccharides on antioxidantenzymes and lipid peroxidation in aged mice[J].J Pharm Bi-omed Anal,2007,43(1):364-370.
• [7]Mano T,Sinohara R,Sawai Y,et al.Changes in lipid peroxi-dation and free radical scavengers in the brain of hyper-and hy-pothyroid aged rats[J].J Endocrinol,1995,147(2):361-365.
• [8]Sawant BU,Nadkarni GD,Thakare UR,et al.Changes in lip-id peroxidation and free radical scavengers in kidney of hypo-thyroid and hyperthyroid rats[J].Indian J Exp Biol,2003,41(11):1 334-1 337.
• [9]许晗,尹伟伟,丁鼎.高山红景天总甙抗衰老作用机理的试验研究[J].浙江中医药大学学报,2011,35(2):248-249;252.
• [10]Yokoyama I,Negita M,Liu DG,et al.Prevention of free-radical induced apoptosis by induction of human recombinantCu,Zn-SOD in pig endothelial cells[J].Transpl Int,2002,15(5):220-225.
• [11]Ferraresi R,Troiano L,Roat E,et al.Essential requirement ofreduced glutathione(GSH)for the anti-oxidant effect of theflavonoid quercetin[J].Free Radic Res,2005,39(11):1 249-1258.
• [12]Gawe S,Wardas M,Niedworok E,et al.Malondialdehyde(MDA)as a lipid peroxidation marker[J].Wiad Lek,2004,57(9/10):453-455.
• [13]Tafazoli S,Wright JS,O'Brien PJ.Prooxidant and antioxidantactivity of vitamin E analogues and troglitazone[J].Chem ResToxicol,2005,18(10):1 567-1 574.